Some did, some didn't.
Not PD, but on this site: MLJ/Archie's Black Hood, in
Top-Notch Comics fought the Skull seven times before the bad guy got the electric chair. Captain Marvel and his family had quite the rogues gallery.
PD: Ace Comics' Magno, in
Super-Mystery Comics fought the Clown often. (Too often, IMO.) Hillman's Air-Boy had Misery & his Air-Tomb. Novelty's Chameleon, in
Target Comics, captured a hypnotist several times, but the jail might as well have had a revolving door.
I'm sure others will come to mind, as soon as I hit send.
